Co-expression of monodehydroascorbate reductase and dehydroascorbate reductase from Brassica rapa effectively confers tolerance to freezing-induced oxidative stress
نویسندگان
چکیده
منابع مشابه
Monodehydroascorbate Reductase from Cucumber
Monodehydroascorbate reductase (EC 1.6.5.4) was purified from cucumber fruit to a homogeneous state as judged by polyacrylamide gel electrophoresis. The cucumber monodehydroascorbate reductase was a monomer with a molecular weight of 47,000. It contained 1 mol of FAD/mol of enzyme which was reduced by NAD(P)H and reoxidized by monodehydroascorbate. The enzyme had an exposed thiol group whose ...
متن کاملIsolation, molecular cloning and expression analysis of Aeluropus littoralis Monodehydroascorbate reductase (MDHAR) gene under salt stress
Plants are naturally exposed to frequent changes in environmental conditions such as salt stress affecting their growth and development, and consequently a considerable reduction in total productivity. Monodehydroascorbate reductase (MDHAR), a key enzyme in ascorbate-glutathione cycle, serves as an important antioxidative enzyme in scavenging of reactive oxygen spices (ROS). In this study, ...
متن کاملAnalysis of nitrate reductase mRNA expression and nitrate reductase activity in response to nitrogen supply
Nitrate is one of the major sources of nitrogen for the growth of plants. It is taken up by plant roots and transported to the leaves where it is reduced to nitrite in the. The main objective of this research was to investigate stimulatory effects of sodium nitrate, potassium nitrate, ammonia and urea on the production/generation of the nitrate reductase mRNA in Triticum aestivum plants. The pl...
متن کاملEnhanced Ascorbic Acid Accumulation through Overexpression of Dehydroascorbate Reductase Confers Tolerance to Methyl Viologen and Salt Stresses in Tomato
As an important antioxidant for plants and humans, L-ascorbic acid (AsA, vitamin C) can scavenge reactive oxygen species (ROS) and can be regenerated from its oxidized form in a reaction catalyzed by dehydroascorbate reductase (DHAR). To analyse the effect of overexpressing DHAR on tomato (Solanum lycopersicum), an expression vector containing potato cytosolic DHAR (DHAR1) or chloroplastic DHAR...
متن کاملisolation, molecular cloning and expression analysis of aeluropus littoralis monodehydroascorbate reductase (mdhar) gene under salt stress
plants are naturally exposed to frequent changes in environmental conditions such as salt stress affecting their growth and development, and consequently a considerable reduction in total productivity. monodehydroascorbate reductase (mdhar), a key enzyme in ascorbate-glutathione cycle, serves as an important antioxidative enzyme in scavenging of reactive oxygen spices (ros). in this study, mdha...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Molecules and Cells
سال: 2013
ISSN: 1016-8478,0219-1032
DOI: 10.1007/s10059-013-0071-4